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Addgene inc sinv 6k

Generation and growth kinetics of <t>SINV</t> <t>6K</t> ion-channel chimeras. ( A ) The amino acid sequences of 6K and 6K mutant chimeras—WT SINV, ∆6K SINV, SINV-ETM, SINV-ETM (N15A), SINV-ETM (V25F), and SINV-ETM (N15A, V25F). The ETM sequence is shown in red with channel-inactivating mutations in blue. ( B ) Plaque morphologies of viruses mentioned in ( A ) grown in BHK-21 cells for 2 days after electroporation. ( C ) Differences in mean plaque diameters of 10 randomly selected plaques for every mutant virus were compared to WT SINV using GraphPad Prism software. Dunnett’s multiple comparisons test as part of one-way ANOVA was used to determine statistical significance with a 95% confidence interval. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 and ns-not significant. ( D ) One-step growth curves of wild-type and channel mutant viruses over 12 hours of infection in BHK-21 cells. Cells were infected at an MOI of 1, and virus-containing media were harvested at given time points to indicate the rate of virus release per hour. Data are representative of two independent experiments. ( E ) Comparison of viral titers and the total number of viral RNA genomes produced by wild-type and mutant viruses 12 hours after electroporation with in vitro transcribed RNA. The experiment was performed in triplicate, and error bars represent the standard deviation (SD). ( F ) One-step growth curves of wild-type and mCherry-tagged SINV and SINV-ETM chimera at an MOI of 2. Data are representative of two independent experiments.

Sinv 6k, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sinv 6k/product/Addgene inc
Average 90 stars, based on 1 article reviews

sinv 6k - by Bioz Stars, 2026-03

90/100 stars

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1) Product Images from "A BSL-2 chimeric system designed to screen SARS-CoV-2 E protein ion channel inhibitors"

Article Title: A BSL-2 chimeric system designed to screen SARS-CoV-2 E protein ion channel inhibitors

Journal: Journal of Virology

doi: 10.1128/jvi.02252-24

Generation and growth kinetics of SINV 6K ion-channel chimeras. ( A ) The amino acid sequences of 6K and 6K mutant chimeras—WT SINV, ∆6K SINV, SINV-ETM, SINV-ETM (N15A), SINV-ETM (V25F), and SINV-ETM (N15A, V25F). The ETM sequence is shown in red with channel-inactivating mutations in blue. ( B ) Plaque morphologies of viruses mentioned in ( A ) grown in BHK-21 cells for 2 days after electroporation. ( C ) Differences in mean plaque diameters of 10 randomly selected plaques for every mutant virus were compared to WT SINV using GraphPad Prism software. Dunnett’s multiple comparisons test as part of one-way ANOVA was used to determine statistical significance with a 95% confidence interval. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 and ns-not significant. ( D ) One-step growth curves of wild-type and channel mutant viruses over 12 hours of infection in BHK-21 cells. Cells were infected at an MOI of 1, and virus-containing media were harvested at given time points to indicate the rate of virus release per hour. Data are representative of two independent experiments. ( E ) Comparison of viral titers and the total number of viral RNA genomes produced by wild-type and mutant viruses 12 hours after electroporation with in vitro transcribed RNA. The experiment was performed in triplicate, and error bars represent the standard deviation (SD). ( F ) One-step growth curves of wild-type and mCherry-tagged SINV and SINV-ETM chimera at an MOI of 2. Data are representative of two independent experiments.

Figure Legend Snippet: Generation and growth kinetics of SINV 6K ion-channel chimeras. ( A ) The amino acid sequences of 6K and 6K mutant chimeras—WT SINV, ∆6K SINV, SINV-ETM, SINV-ETM (N15A), SINV-ETM (V25F), and SINV-ETM (N15A, V25F). The ETM sequence is shown in red with channel-inactivating mutations in blue. ( B ) Plaque morphologies of viruses mentioned in ( A ) grown in BHK-21 cells for 2 days after electroporation. ( C ) Differences in mean plaque diameters of 10 randomly selected plaques for every mutant virus were compared to WT SINV using GraphPad Prism software. Dunnett’s multiple comparisons test as part of one-way ANOVA was used to determine statistical significance with a 95% confidence interval. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 and ns-not significant. ( D ) One-step growth curves of wild-type and channel mutant viruses over 12 hours of infection in BHK-21 cells. Cells were infected at an MOI of 1, and virus-containing media were harvested at given time points to indicate the rate of virus release per hour. Data are representative of two independent experiments. ( E ) Comparison of viral titers and the total number of viral RNA genomes produced by wild-type and mutant viruses 12 hours after electroporation with in vitro transcribed RNA. The experiment was performed in triplicate, and error bars represent the standard deviation (SD). ( F ) One-step growth curves of wild-type and mCherry-tagged SINV and SINV-ETM chimera at an MOI of 2. Data are representative of two independent experiments.

Techniques Used: Mutagenesis, Sequencing, Electroporation, Virus, Software, Infection, Comparison, Produced, In Vitro, Standard Deviation

SINV 6K and SARS-CoV-2 E proteins bind to amiloride derivatives. UV-Vis spectra of ( A ) HMA, EIPA, and DMA. UV-Vis spectra of compounds bound to ( B ) SINV 6K, ( C ) SARS-CoV-2 E, and ( D ) EGFP proteins. Blank refers to the absorbance of the dialysis buffer after unbound compounds have been removed from each sample. The data shown represent two independent experiments.

Figure Legend Snippet: SINV 6K and SARS-CoV-2 E proteins bind to amiloride derivatives. UV-Vis spectra of ( A ) HMA, EIPA, and DMA. UV-Vis spectra of compounds bound to ( B ) SINV 6K, ( C ) SARS-CoV-2 E, and ( D ) EGFP proteins. Blank refers to the absorbance of the dialysis buffer after unbound compounds have been removed from each sample. The data shown represent two independent experiments.

Techniques Used:

Inhibition of SINV and SINV-ETM mutant virus infection by amilorides and amantadine in BHK cells at MOI of 1.0 and 12 h incubation. BHK cells were treated with indicated concentrations of ( A, E ) HMA, ( B, F ) EIPA, ( C, G ) DMA, and ( D, H ) amantadine and infected with ( A–D ) mCherry E2 WT SINV (green), mCherry E2 Δ6K SINV (pink) and ( E–H ) mCherry E2 SINV-ETM (orange), ETM (N15A) (blue), ETM (V25F) (red), ETM (N15A, V25F) (purple) viruses at an MOI of 1.0 for 12 hours. ( I ) BHK-21 cells were infected with untagged WT SINV, ∆6K SINV, and SINV-ETM chimera at an MOI of 1.0 treated with indicated concentrations of inhibitors and plaqued after 12 hours.

Figure Legend Snippet: Inhibition of SINV and SINV-ETM mutant virus infection by amilorides and amantadine in BHK cells at MOI of 1.0 and 12 h incubation. BHK cells were treated with indicated concentrations of ( A, E ) HMA, ( B, F ) EIPA, ( C, G ) DMA, and ( D, H ) amantadine and infected with ( A–D ) mCherry E2 WT SINV (green), mCherry E2 Δ6K SINV (pink) and ( E–H ) mCherry E2 SINV-ETM (orange), ETM (N15A) (blue), ETM (V25F) (red), ETM (N15A, V25F) (purple) viruses at an MOI of 1.0 for 12 hours. ( I ) BHK-21 cells were infected with untagged WT SINV, ∆6K SINV, and SINV-ETM chimera at an MOI of 1.0 treated with indicated concentrations of inhibitors and plaqued after 12 hours.

Techniques Used: Inhibition, Mutagenesis, Virus, Infection, Incubation

Time of inhibitor addition assay at high MOI. ( A ) Schematic representation of the time of addition assay protocol. ( B ) Time of addition assay in BHK-21 cells with channel inhibitors. Cells were infected with either mCherry-E2-tagged SINV or SINV-ETM virus at t = 0 with a high MOI of 10. 25 µM HMA, 25 µM EIPA, 50 µM DMA, and 500 µM Amantadine were added to the cells at the indicated time points. Cells were fixed and imaged at 15 hpi at 4× magnification. The fluorescence intensity signal (TRITC channel) was measured for each sample relative to the DMSO-control signal and plotted for graphical representation. Error bars represent the standard error of the mean (SEM) of two independent experiments. Statistical significance was determined for each time point between inhibitor-treated and DMSO control values by two-tailed unpaired t-test with a 95% confidence interval. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 and ns-not significant.

Figure Legend Snippet: Time of inhibitor addition assay at high MOI. ( A ) Schematic representation of the time of addition assay protocol. ( B ) Time of addition assay in BHK-21 cells with channel inhibitors. Cells were infected with either mCherry-E2-tagged SINV or SINV-ETM virus at t = 0 with a high MOI of 10. 25 µM HMA, 25 µM EIPA, 50 µM DMA, and 500 µM Amantadine were added to the cells at the indicated time points. Cells were fixed and imaged at 15 hpi at 4× magnification. The fluorescence intensity signal (TRITC channel) was measured for each sample relative to the DMSO-control signal and plotted for graphical representation. Error bars represent the standard error of the mean (SEM) of two independent experiments. Statistical significance was determined for each time point between inhibitor-treated and DMSO control values by two-tailed unpaired t-test with a 95% confidence interval. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 and ns-not significant.

Techniques Used: Infection, Virus, Fluorescence, Control, Two Tailed Test

Image Search Results

Journal: Journal of Virology

Article Title: A BSL-2 chimeric system designed to screen SARS-CoV-2 E protein ion channel inhibitors

doi: 10.1128/jvi.02252-24

Figure Lengend Snippet: Generation and growth kinetics of SINV 6K ion-channel chimeras. ( A ) The amino acid sequences of 6K and 6K mutant chimeras—WT SINV, ∆6K SINV, SINV-ETM, SINV-ETM (N15A), SINV-ETM (V25F), and SINV-ETM (N15A, V25F). The ETM sequence is shown in red with channel-inactivating mutations in blue. ( B ) Plaque morphologies of viruses mentioned in ( A ) grown in BHK-21 cells for 2 days after electroporation. ( C ) Differences in mean plaque diameters of 10 randomly selected plaques for every mutant virus were compared to WT SINV using GraphPad Prism software. Dunnett’s multiple comparisons test as part of one-way ANOVA was used to determine statistical significance with a 95% confidence interval. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 and ns-not significant. ( D ) One-step growth curves of wild-type and channel mutant viruses over 12 hours of infection in BHK-21 cells. Cells were infected at an MOI of 1, and virus-containing media were harvested at given time points to indicate the rate of virus release per hour. Data are representative of two independent experiments. ( E ) Comparison of viral titers and the total number of viral RNA genomes produced by wild-type and mutant viruses 12 hours after electroporation with in vitro transcribed RNA. The experiment was performed in triplicate, and error bars represent the standard deviation (SD). ( F ) One-step growth curves of wild-type and mCherry-tagged SINV and SINV-ETM chimera at an MOI of 2. Data are representative of two independent experiments.

Article Snippet: Bacterial clones of full-length SINV 6K, SARS-CoV-2 E, EGFP, and E mutant proteins (N15A, V25F, and N15A, V25F double mutant) in pET His6 MBP N10 TEV (Addgene #29706 ) were used for bacterial expression and purification.

Techniques: Mutagenesis, Sequencing, Electroporation, Virus, Software, Infection, Comparison, Produced, In Vitro, Standard Deviation

Journal: Journal of Virology

Article Title: A BSL-2 chimeric system designed to screen SARS-CoV-2 E protein ion channel inhibitors

doi: 10.1128/jvi.02252-24

Figure Lengend Snippet: SINV 6K and SARS-CoV-2 E proteins bind to amiloride derivatives. UV-Vis spectra of ( A ) HMA, EIPA, and DMA. UV-Vis spectra of compounds bound to ( B ) SINV 6K, ( C ) SARS-CoV-2 E, and ( D ) EGFP proteins. Blank refers to the absorbance of the dialysis buffer after unbound compounds have been removed from each sample. The data shown represent two independent experiments.

Techniques:

Journal: Journal of Virology

Article Title: A BSL-2 chimeric system designed to screen SARS-CoV-2 E protein ion channel inhibitors

doi: 10.1128/jvi.02252-24

Figure Lengend Snippet: Inhibition of SINV and SINV-ETM mutant virus infection by amilorides and amantadine in BHK cells at MOI of 1.0 and 12 h incubation. BHK cells were treated with indicated concentrations of ( A, E ) HMA, ( B, F ) EIPA, ( C, G ) DMA, and ( D, H ) amantadine and infected with ( A–D ) mCherry E2 WT SINV (green), mCherry E2 Δ6K SINV (pink) and ( E–H ) mCherry E2 SINV-ETM (orange), ETM (N15A) (blue), ETM (V25F) (red), ETM (N15A, V25F) (purple) viruses at an MOI of 1.0 for 12 hours. ( I ) BHK-21 cells were infected with untagged WT SINV, ∆6K SINV, and SINV-ETM chimera at an MOI of 1.0 treated with indicated concentrations of inhibitors and plaqued after 12 hours.

Techniques: Inhibition, Mutagenesis, Virus, Infection, Incubation

Journal: Journal of Virology

Article Title: A BSL-2 chimeric system designed to screen SARS-CoV-2 E protein ion channel inhibitors

doi: 10.1128/jvi.02252-24

Figure Lengend Snippet: Time of inhibitor addition assay at high MOI. ( A ) Schematic representation of the time of addition assay protocol. ( B ) Time of addition assay in BHK-21 cells with channel inhibitors. Cells were infected with either mCherry-E2-tagged SINV or SINV-ETM virus at t = 0 with a high MOI of 10. 25 µM HMA, 25 µM EIPA, 50 µM DMA, and 500 µM Amantadine were added to the cells at the indicated time points. Cells were fixed and imaged at 15 hpi at 4× magnification. The fluorescence intensity signal (TRITC channel) was measured for each sample relative to the DMSO-control signal and plotted for graphical representation. Error bars represent the standard error of the mean (SEM) of two independent experiments. Statistical significance was determined for each time point between inhibitor-treated and DMSO control values by two-tailed unpaired t-test with a 95% confidence interval. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 and ns-not significant.

Techniques: Infection, Virus, Fluorescence, Control, Two Tailed Test

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